Genesis 4:1 "And the man knew Eve his wife. And she conceived and bore Cain [possession], and said, I have gotten a man: Yehovah." (Adam and Eve understood that the Messiah would be God in the flesh, but Cain being anything but God was the first murder of man and of the seed of Adam, not of the Holy Spirit as in the virgin birth of Jesus)

Isaiah 7:14 "Therefore Yehovah Himself will give you a sign: Behold, a virgin¹⁷¹ will be with child and bear a son, and she will call His name Immanuel."

Matthew 1:18-25 "Now the birth of Jesus Christ was in this way: Mary, the mother of Him, being betrothed to Joseph before they came together was found to be pregnant by the Holy Spirit.  But, her husband Joseph, being just, and not willing to expose her by way of example, purposed to put her away secretly.  And as he was thinking on these things, behold, an angel of the Lord was seen by him in a dream, saying, 'Joseph, son of David, do not fear to take Mary as your wife, for that which is conceived in her is by the Holy Spirit.  And she will bear a Son; and you shall call His name Jesus, for it is He who will save His people from their sins."  Now all this took place that what was spoken by the Lord through the prophet might be fulfilled, saying, "Behold, the virgin shall be with child, and shall bear a son, and they shall call his name Immanuel," which translated means, "God with us."'  And Joseph arose from his sleep, and did as the angel of the Lord commanded him, and took her as his wife, and kept her a virgin until she gave birth to a Son; and he called His name Jesus." (Matthew 1:18-25)